Sorghum, a drought-tolerant crop, may serve as a promising forage alternative to corn in water-scarce regions. The study objective was to assess the effect of replacing conventional (nonbrown midrib) corn silage (CCS) with brown midrib male-sterile sorghum silage (BMR-MS-SS) at replacement rates of 0% (control), 25% (25%-BMR-MS-SS), and 50% (50%-BMR-MS-SS) on apparent total-tract nutrient digestibility (TTD), DMI, and ECM production. Compared with the control, protein concentrates and grass hay were decreased and dry ground corn increased in 25%-BMR-MS-SS and 50%-BMR-MS-SS to maintain similar nutrient composition among diets.

The objective of this observational study was to investigate the association between the duration of labor, calving assistance, and uterine diseases, as well as estimate a reference time to be used as a guideline for providing calving assistance based on the health outcome of metritis. Holstein cows (n = 496) were enrolled approximately 3 wk before their expected calving date. Cameras were used to record calving behavior of a subset of cows (n = 286), where the occurrence of calving assistance (forced extraction lasting a minimum of 5 s), calving location, and pen movement during labor were recorded.

This study examined national patterns in Taiwan's frozen dairy cattle semen imports from 2020 to 2024 using official import clearance records issued by the Bureau of Animal and Plant Health Inspection and Quarantine. A total of 643 individual Holstein sires were identified across all shipments during the 5-yr period. Annual import volumes, semen type distributions, and sire-specific usage patterns were analyzed to characterize changes in breeding strategies. Genetic indices for imported sires were obtained from the Council on Dairy Cattle Breeding August 2025 evaluation and linked with import data to assess how sire characteristics corresponded to importer preferences.

Dairy cow grazing is widely appreciated by society due to its perceived benefits for animal welfare and health. However, concerns about dairy cow health are also cited as reasons for adopting year-round confinement systems. Comprehensive assessments on the relation of grazing intensity and duration (GID) with dairy cow production and health under practical conditions are lacking. This exploratory, longitudinal observational field study assessed the association between grazing parameters and production and health indicators in 70 commercial Dutch dairy herds during the 2023 and 2024 grazing seasons.

This study evaluated the production performance, milk fatty acid profile, nutrient digestibility, and enteric methane (CH4) emissions of high-producing dairy cows fed high-forage (HF; 52% forage) or low-forage (LF; 37% forage) diets containing triticale silages harvested at either the boot (BT) or soft-dough (SFT) stage. The BT stage silage contained 16.7% CP, 51.1% NDF, 35.0% ADF, 3.7% ADL, and 2.2% starch, whereas the SFT stage silage contained 8.7% CP, 62.6% NDF, 46.1% ADF, 6.4% ADL, and 4.6% starch.

With increasing consumer demand for goat milk, accelerating the breeding of elite dairy goats through livestock embryo engineering has become imperative. Elucidating estrogen's molecular mechanism in primordial follicle activation is crucial for improving in vitro embryo production to enhance breeding efficiency. This study aimed to elucidate the molecular mechanisms by which estrogen regulates the activation of primordial follicles in goats. By supplementing the ovarian cortical fragment culture system with estrogen and small molecular reagents, and utilizing experimental techniques including hematoxylin and eosin staining, immunohistochemical staining, immunofluorescence staining, RT quantitative PCR, Western blot analysis, and ELISA, we demonstrated that the protein levels of phosphorylated (p)-mTOR, p-rpS6, and KITL were significantly higher in granulosa cells of growing follicles than in those of primordial follicles in goat ovaries.

This study used a data fusion approach, combining rumen and milk metabolomic profiling, to investigate the biochemical effects of 3-nitrooxypropanol (3-NOP), an effective methanogenesis inhibitor. A controlled feeding trial was conducted in 2 phases (i.e., summer and fall) with Holstein dairy cows (n = 30/phase) fed a diet supplemented for 9 wk with 60 mg/kg DM of 3-NOP or a placebo. Rumen fluid and milk samples were collected at the end of the experimental phases and analyzed using ultra-HPLC coupled with high-resolution mass spectrometry.

This study examined the effects of supplementation with increasing levels of RUP on performance, nitrogen metabolism, and mammary gland development in grazing dairy heifers. Twenty-eight 5/8 crossbred Holstein × Gyr dairy heifers (initial BW of 278 ± 50.4 kg) grazed on Megathyrsus maximus cv. BRS Quênia grass pastures for an experimental period of 84 d, divided into 4 subperiods of 21 d each. The experiment followed a completely randomized design in a 4 × 3 factorial arrangement, with 4 supplementation strategies (control [CON], or supplementation with 30% [RUP30], 48% [RUP48], or 66% [RUP66] RUP) and 3 initial BW classes (light, medium, and heavy).