Holstein cow milk–derived Lactobacillus plantarum L19 alleviates heat stress–induced liver injury in mice by modulating gut microbiota. By Wang et al., page 17. This study is the first to demonstrate that Lactiplantibacillus plantarum L19, a lactic acid bacterium isolated from Holstein cow milk, effectively alleviates heat stress–induced liver injury in mice. It broadens the application of cow milk–derived lactic acid bacteria, offers new strategies for managing heat-induced liver injury in humans and animals, and provides a scientific basis for using lactic acid bacteria in related disease prevention.

This article contained an incorrect technical explanation for the discrepancy observed between 2 statistical models (ICMSF and JERMA), in which the authors initially attributed the difference to how the models account for lot size.

In this article, metabolic heat production (MHP) was incorrectly calculated on a mass basis (g/d) rather than on a volume basis (L/d). The authors have recalculated all affected values, and the updated values appear in boldface in the article text and Table 2 below. The correct versions of Figure 7 and Figure 8 also appear below.

The editor in chief elected to retract this article (Ayivi et al., 2022a) because it duplicates significant parts of a paper that had already appeared in the Journal of Dairy Research (Ayivi et al., 2022b).

I am incredibly honored to be the new Editor in Chief of the Journal of Dairy Science, and would like to thank Paul Kononoff for his leadership of the journal over the past 6 yr. He has truly led the journal during a time in which science and publishing have been accelerating in new ways, requiring foresight and rapid adaptation to new challenges, and leaving the journal in the best place it has ever been. I have been working with Paul over the past few months to ensure a smooth transition, with the hope of continuing the momentum, growth, and success of our journal.

The list of standard abbreviations for JDS is available at adsa.org/jds-abbreviations-24. Nonstandard abbreviations are available in the Notes.

Staphylococcus aureus, a prominent global foodborne pathogen, frequently triggers epidemics with severe public health impacts. Timely and reliable detection of S. aureus is crucial for mitigating the disease burden in low- and middle-income countries. However, conventional laboratory-based detection methods remain impractical in resource-limited settings, highlighting the urgent need for accessible point-of-care solutions. Here, we present an inner-outer-tube (IOT) assay that synergistically integrates the polymerase spiral amplification (PSR) technology for enhanced sensitivity with the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated 12a (Cas12a) system for sequence-specific identification.

Mastitis represents one of the most formidable challenges in modern dairy farming, posing significant threats to individual cow health and causing substantial economic losses throughout the dairy production chain. Traditional disease diagnosis methods are often reactive and costly, creating an urgent need for advanced predictive technologies. To address these issues, we proposed a novel machine learning–based mastitis prediction system that breaks through conventional diagnostic paradigms by deeply integrating data science with veterinary medicine.

Excessive lipolysis and inflammatory response are critically involved in the pathogenesis of ketosis in periparturient dairy cows. Evidence has been growing for participation of the growth hormone (GH) in the metabolic regulation of adipose tissue. However, the potential role of GH in promoting lipolysis and proinflammatory signaling activation in bovine adipocytes remains to be elucidated. The objective of this study was to investigate the regulatory effects of GH on the lipolysis and inflammatory response of bovine adipocytes.

This study investigated the effects of varying fat levels and ratios of coconut-to-palm fat in milk replacer on solid feed intake and performance of male Holstein calves fed a high milk replacer allowance. Calves were individually housed and enrolled in a 91-d (study 1; n = 128) and 84-d (study 2; n = 128) experiment at the same research facility divided into 3 phases: preweaning (1–42 d), weaning (43–63 d), and postweaning (64-study end). In study 1, calves were randomly assigned to 1 of the 3 treatments, consisting of a milk replacer containing a spray-dried blend of 20% coconut-to-80% palm fat with: 1) low fat (17%; LF-17%; n = 42; BW = 47.64 ± 2.62 kg), 2) moderate fat (23%; MF-23%; n = 43; BW = 47.69 ± 3.42 kg), and 3) high fat (29%; HF-29%; n = 41; BW = 47.04 ± 3.74 kg) in relation to 26% CP level.

The extent of genetic variability in fatty acids in bovine milk has, to date, generally focused on its concentration in either milk or milk fat. Selection for ratio traits, such as fatty acid concentration, is statistically and biologically problematic because it can distort relationships between component traits and lead to unintended genetic responses. The objective of this study was to explore the degree of genetic variability in the total yield of individual fatty acids, including when adjusted to a common fat yield.

Replacing hexane with 2-methyloxolane (MeOx) for defatting soybean meal (SBM) requires adaptations of the SBM production process. These modifications may increase the concentration of Maillard reaction products and reduce the residual oil content in MeOx-defatted SBM compared with hexane-defatted SBM. In addition, despite desolventization, solvent residues may still be present in the SBM when fed to livestock. This study aims to ensure that the replacement of hexane with MeOx for defatting SBM does not affect the ruminal and milk fatty acid profiles nor the liver activity, liver functionality, and inflammatory status in dairy cows.

Three studies evaluated the effects of a Bacillus-based direct-fed microbial (DFM) on ex vivo fermentation traits, as well as on performance of lactating dairy cows. In experiment 1, rumen-fistulated lactating Holstein-Friesian cows (6.6 ± 2.3 parity; 630 ± 37.2 kg BW; 18.9 ± 3.35 kg milk/d) were fed an unsupplemented ration (CON; n = 5) or supplemented with 3 g/cow per d of a Bacillus-based DFM (BAC; n = 5) for 14 d before rumen sampling and subsequent ex vivo evaluation of fermentation characteristics of 2 different substrates (vetch hay [Vicia sativa] and perennial ryegrass herbage [Lolium perenne L.]).

Accurate local ancestry (LA) inference is important for genomic evaluations in admixed dairy cattle. This study had 2 main objectives: to compare the performance of 3 LA inference software: AllOr, BreedOrigin, and ChromoPainter, and to evaluate the impact of LA inference errors on genomic prediction accuracy. Two simulated admixed populations were used: a structured DairyCross population created through three-way rotational crossbreeding and a Red Dairy cattle (RDC) population representing historical admixture among multiple breeds.

The gastrointestinal tract (GIT) plays a crucial role in maintaining overall health and performance in ruminants, with the rumen functioning as fermentation chamber for plant biomass. Intestinal epithelial cells are exposed to billions of microorganisms that significantly affect the epithelial barrier by activating complex metabolic and immune pathways. The interaction between microbiota and host is mediated by signaling compounds such as metabolites and peptides, and also extracellular vesicles (EV).

The purpose of this study was to use a secondary analysis to investigate the use of feeding high and low-energy diets to alter BCS at dry-off, and to assess the subsequent changes in BCS on feed intake, calcemia, and assessment of immune function, including inflammation, during the periparturient period. Multiparous Holstein lactating dairy cows at 150 d of gestation were blocked by expected calving date and randomly assigned to receive one of 2 dietary treatments during late lactation until dry-off, aiming to maintain or decrease BCS.

Dairy cows are highly motivated to use grooming brushes, and their use promotes expression of natural behavior. Although brushes are increasingly common on commercial dairy farms, little is known about how their mechanical properties, specifically swingability and rotation, influence cattle preferences for brush use. This study investigated adult dairy cow preferences for 3 brushes identical in physical and visual features but differing mechanically: (1) swinging and rotating, (2) swinging-only, and (3) stationary (neither swinging nor rotating).

Lactiplantibacillus plantarum is a widely studied lactic acid bacterium with important roles in gut health and fermented food production. Accurate quantification of viable populations is crucial for evaluating the efficacy of probiotics and ensuring product quality. In this study, RNA sequencing was applied for the first time to systematically screen stable reference genes (RG) in L. plantarum for reverse-transcription quantitative PCR (RT-qPCR) under 4 distinct fermentation conditions. Among 3,092 genes analyzed, 22 candidates exhibiting CV values below 0.1 were identified.