Forty-eight multiparous Holstein cows were used in a randomized complete block design and assigned to one of 4 treatments in a 2x2 factorial arrangement of treatments to determine the effects of supplemental palmitic acid (C16:0) and chromium (Cr) on production responses of early-lactation cows. During the fresh period (FR; 1–24 d in milk), cows were fed one of 4 treatments: (1) a diet containing no supplemental C16:0 or Cr (CON); (2) diet supplemented with an 85% C16:0-enriched supplement (PA); (3) diet supplemented with Cr-propionate (CR); and (4) diet supplemented with a C16:0-enriched supplement and Cr-propionate (PACR).
The endocannabinoid system (ECS) is involved in the regulation of energy metabolism, immune function and reproduction in mammals. The ECS is consisted of the endocannabinoid (eCB) ligands, enzymes, and cannabinoid receptors. In mammals, the cannabinoid-1 receptor (CB1/CNR1) is expressed in the central nervous system and in peripheral tissues; and its activation increases anabolic processes. The cannabinoid-2 receptor (CB2/CNR2) is most highly expressed in immune cells, and its activation exerts mainly anti-inflammatory effects.
There is currently a lack of research examining the association between the consumption of different dairy products and type 2 diabetes (T2D) in East Asian populations. To address this gap, the present study employs Mendelian randomization (MR) to investigate the potential impact of 3 different types of milk consumption (including whole milk, semi-skim milk, and skim milk) on the risk of developing T2D. The results indicate that both whole milk and skim milk are associated with an increased risk of T2D (whole milk: OR = 1.022, 95% confidence interval [CI]: 1.001–1.044, P = 0.042, skim milk: OR = 1.023, 95% CI: 1.007–1.039, P = 0.005).
Experimental objectives were to create a chronic inflammatory model to evaluate the effects of persistent immune activation on metabolism, inflammation, and productivity in lactating dairy cows. Twelve lactating Holstein cows (631 ± 16 kg BW; 124 ± 15 DIM) were enrolled in a study with 2 experimental periods (P); during P1 (5 d), cows were fed ad libitum and baseline data were obtained. At the initiation of P2 (7 d), cows were assigned to 1 of 2 treatments: 1) saline-infused and pair-fed (PF; 5 mL intravenously (IV) sterile saline on d 1, 3, and 5; n = 6) or 2) lipopolysaccharide infused and ad libitum-fed (LPS; 0.2, 0.8, and 1.6 µg LPS/kg BW IV on d 1, 3, and 5, respectively; n = 6).
The increased use of dairy fat in various applications is facilitated by its fractionation into hard and liquid fractions. Herein, the fractionation of bovine ghee and buffalo ghee was investigated, and triacylglycerol (TAG) profiles of milk fat fractions and 2 categories of infant formulas fat were quantified by using ultra-performance convergence chromatography quadrupole time-of-flight mass spectrometry (UPC2-Q-TOF-MS) using carbon dioxide as the mobile phase. Furthermore, the thermal behavior of the different samples was evaluated, and tocopherols content was also quantified.
The objective of this study was to investigate the effect of dynamic pulsation settings that increased the open phase and reduced the closed phase of pulsation during the peak milk flow period together with increasing the milk flow rate switch-point for cluster detachment on milking duration and teat condition after milking. The present study filled current gaps in knowledge by informing on the effects of both milk flow rate switch-points and dynamic pulsation together in one experiment, while presenting data on milking performance, strip milk, teat condition and vacuum levels in the cluster during milking.
Bovine respiratory disease (BRD) is one of the most common diseases observed in dairy calves in both the pre- and post-weaning periods. Despite its common occurrence, there has been no formal synthesis (e.g., scoping review) of the scientific literature surrounding diagnosing BRD in dairy calves. As such, the objective of this scoping review was to describe what case definitions for diagnosing BRD in dairy calves have been used in research. An initial search of peer-reviewed journals and the gray literature yielded 12,001 unique articles, of which 307 records containing 358 case definitions were included.
Provision of supplemental concentrate in an automated milking system (AMS) is commonly used to encourage voluntary attendance, however, the motivation to voluntarily milk is highly variable between cows. The objectives of this study were to determine if dairy cow personality is associated with: 1) their short-term response to changes in factors believed to motivate voluntary AMS visits such as udder pressure and provision of supplemental feed (modulated by longer milking intervals or removal of AMS concentrate, respectively); and 2) their milking activity, production, and feeding behavior after returning to pre-treatment AMS milking interval and concentrate feed settings (i.e., behavioral flexibility).
The rumen microbiome is essential for breaking down indigestible plant material, supplying ruminants with most of their metabolizable energy and protein. While research has primarily focused on bacteria and archaea, protozoa and viruses (phages) have only gained attention in recent years. Protozoa contribute to feed digestion and fermentation, but as predators, they regulate microbial populations by lysing large quantities of microbial cells (the primary protein source for ruminants) and influence the amount of microbial protein reaching the small intestines, along with other mechanisms of interactions.
Anaerobic gut fungi (AGF) were the last phylum to be identified within the rumen microbiome and account for 7–9% of microbial biomass. They produce potent lignocellulases that degrade recalcitrant plant cell walls, and rhizoids that can penetrate the cuticle of plant cells, exposing internal components to other microbiota. Interspecies H2 transfer between AGF and rumen methanogenic archaea is an essential metabolic process in the rumen that occurs during the reduction of CO2 to CH4 by methanogens.
High moisture Mozzarella cheese is particularly popular because of its freshness and milky flavor, however the difference in aroma compound composition between high moisture Mozzarella cheese made from bovine (BOC) and buffalo milk (BUC) remains unclear. Herein, the volatile compounds of 2 kinds of Mozzarella cheese were qualitatively and quantitatively analyzed by SPME-GC × GC-O-TOFMS, SPME-Arrow-GC-MS and GC-IMS for the first time. A total of 139 volatile compounds were identified (69 aroma active compounds were sniffed), of which 106 were identified in BOC and 96 in BUC.
Yak milk is a potential nutrient for improving osteoporosis. However, the effect of yak milk on the expression of Ca2+ion channel TRPV5 during osteoclast (OC) differentiation is still unclear. This study used ruthenium red as a control to investigate the effect of yak milk on osteoclast differentiation and activity. TRAP staining and bone resorption pit experiments showed that yak milk inhibited osteoclast differentiation and bone resorption activity in a dose-dependent manner. In addition, yak milk can inhibit osteoclast activity by inhibiting the expression of TRPV5.
We aimed to evaluate the effects of prepartum supplementation of different I sources (Ascophyllum nodosum [ASCO] meal and ethylenediamine dihydroiodide [EDDI]) on colostrum yield of cows, and blood concentrations of glucose, BHB, and thyroid hormones and growth of dairy calves. Forty multiparous Holstein cows were blocked by lactation number and expected calving date and assigned to 1 of 4 treatments 28 d before parturition: (1) EDDI supplemented (11 mg/d) to a basal diet to meet the NRC (2001) I concentration of 0.5 mg of I/kg of DMI (control = CON [0 g/d of ASCO meal]; actual I concentration = 0.68 mg/kg of DMI), (2) CON plus 57 g/d of ASCO meal (low seaweed = LSW), (3) CON plus 113 g/d of ASCO meal (high seaweed = HSW), or (4) CON plus 151.3 mg/d of EDDI formulated to match the amount of I provided by HSW (HEDDI).
Clinical mastitis, a prevalent production disease in the dairy industry, causes significant pain and swelling in dairy cows' udders. While previous research highlights a symbiotic relationship between humans and animals, particularly in terms of health, this study investigates how animal health, specifically clinical mastitis, influences farmers' well-being. Acknowledging farmers' pivotal role in mitigating animal health problems, we examined the human-animal relationship by exploring how dairy cow health relates to the psychological well-being of dairy farmers.
Our aim was to determine the effects of P intake on P balance, serum parathyroid hormone (PTH) levels and bone resorption during the final 4 weeks prepartum and the first 8 weeks of lactation. Sixty pregnant multiparous Holstein Friesian dairy cows were assigned to a randomized block design with repeated measurements and dietary treatments arranged according to a 2 × 2 factorial design. The experimental diets contained 3.6 (high phosphorus, HP) or 2.2 (low phosphorus, LP) g P/kg DM during the dry period (Dry-HP and Dry-LP, respectively) and 3.8 or 2.9 g P/kg DM during 56 d after calving (Lac-HP and Lac-LP, respectively).
Activation of the endocannabinoid system (ECS) elicits negative effects on the reproductive system in mammals. Omega-3 (n-3) fatty acid (FA) supplementation lowers ECS activation and has anti-inflammatory effects. Thus, we hypothesized that supplementing cows with n-3 FA will downregulate components of the ECS and immune system in preovulatory follicles and in the endometrium. Twenty-four multiparous Holstein dairy cows were supplemented from d 256 of pregnancy to d 70 postpartum as follows: (i) control (CTL; n = 12) prepartum with 250 g/d/cow calcium salts of FA and postpartum at 1.6% of the diet (DM basis); (ii) FLX (n = 12) prepartum with 700 g/d/cow of extruded flaxseed supplement rich in α-linolenic acid (ALA, C18:3n-3), and postpartum at 6.4% of diet (DM basis) of the same supplement.
The first days and weeks on the job set the course for a new dairy farm employee. This project involved an educational intervention to increase the use of new employee onboarding practices in dairy farms and analyzes the resulting effects on (1) levels of onboarding practice use, (2) manager perceptions of employee performance, (3) manager satisfaction with the onboarding program, (4) manager concerns about compliance with state and federal employment regulations, and (5) employee turnover. Onboarding advisors (educators and consultants) provided templates, examples, and intensive facilitation directly with farm managers to learn and adopt onboarding practices.
