Identifying physiological determinants of dairy cow survival and their potential modulation by parity may reveal opportunities to improve herd health and longevity. This multisite, prospective, observational study investigated culling and mortality hazards using targeted lipidomic and standard metabolite assays. Blood samples, stratified by parity, were collected from 2 cow cohorts (1) dry and (2) peak-milk, from across 29 commercial Australian farms (14 pasture-based, 15 confinement-based). There were 717 nonlactating, late-pregnant, dry cows (∼27 d prepartum) and 794 peak-milk cows (∼58 DIM) sampled.

This study investigated the effects of TMR moisture content and forage source on feed intake, growth performance, nutrient digestibility, rumen fermentation, and blood metabolites in Holstein calves. Forty-eight calves (24 males, 24 females; initial BW: 39.8 ± 2.18 kg) were used in a 2 × 2 factorial arrangement of treatment with the factors being moisture content of TMR (10% or 50%) and forage source (alfalfa hay [AH] or wheat straw [WS]). Calves were randomly assigned to one of the 4 treatments, including (1) a TMR containing WS with 10% (WS10) or (2) 50% (WS50) moisture content, and TMR diet containing (3) AH with 10% (AH10) or (4) 50% (AH50) moisture content.

Fermented milk is a widely popular probiotic food. In addition to starter cultures, its production process is often enhanced with specific probiotic strains, such as Bifidobacterium spp., Lactiplantibacillus plantarum, and Lacticaseibacillus rhamnosus, although the particular strains employed vary among different products. Because the efficacy of probiotics is strain-specific, determining the composition and abundance of probiotic strains in fermented milk is critical for the quality assessment and regulation of these products.

Methane (CH4) sniffers integrated into automated milking systems (AMS) are increasingly deployed to collect high-resolution, large-scale CH4 emission data from dairy cows. The objective of this study was to assess how sniffer nozzle placement in AMS affects enteric CH4 measurements in dairy cows. Nine CH4 sniffer units (SimpleScan; C-Lock Inc.) were installed inside the AMS (DeLaval VMS) to measure enteric CH4 concentration (ppm), with intake nozzles arranged in a 3 × 3, 15-cm grid above the feed bin.

With the world's population expected to reach 9 billion by 2050, there are growing concerns about the increasing inclusion of human-edible (HE) feedstuffs in the diets of ruminant livestock. Two experiments (a smaller scale study involving late lactation cows and a larger scale study involving early lactation cows) examined the impact of varying the human-edible fraction (HEF) of the concentrate on the performance of dairy cows offered grass silage–based diets. Changes in HEF were achieved by replacing the cereal and soybean meal component of the concentrate with byproduct feed ingredients, including sugar beet pulp, rapeseed meal, and distillers dark grains.

Lameness is a major welfare and productivity concern in dairy herds. This study investigated the influence of animal traits (parity, BCS) and environmental factors (farm, season) on locomotion score (LS) in lactating cows and assessed the impact of lameness on milking parameters using data from 3 Italian farms equipped with automatic milking systems (AMS). A total of 323 cows were evaluated biweekly for LS and BCS over 7 mo. The AMS data (n = 42,569 observations) were collected and analyzed with linear mixed models to assess relationships between LS and milking parameters.

Holstein cow milk–derived Lactobacillus plantarum L19 alleviates heat stress–induced liver injury in mice by modulating gut microbiota. By Wang et al., page 17. This study is the first to demonstrate that Lactiplantibacillus plantarum L19, a lactic acid bacterium isolated from Holstein cow milk, effectively alleviates heat stress–induced liver injury in mice. It broadens the application of cow milk–derived lactic acid bacteria, offers new strategies for managing heat-induced liver injury in humans and animals, and provides a scientific basis for using lactic acid bacteria in related disease prevention.

This article contained an incorrect technical explanation for the discrepancy observed between 2 statistical models (ICMSF and JERMA), in which the authors initially attributed the difference to how the models account for lot size.

In this article, metabolic heat production (MHP) was incorrectly calculated on a mass basis (g/d) rather than on a volume basis (L/d). The authors have recalculated all affected values, and the updated values appear in boldface in the article text and Table 2 below. The correct versions of Figure 7 and Figure 8 also appear below.

The editor in chief elected to retract this article (Ayivi et al., 2022a) because it duplicates significant parts of a paper that had already appeared in the Journal of Dairy Research (Ayivi et al., 2022b).

I am incredibly honored to be the new Editor in Chief of the Journal of Dairy Science, and would like to thank Paul Kononoff for his leadership of the journal over the past 6 yr. He has truly led the journal during a time in which science and publishing have been accelerating in new ways, requiring foresight and rapid adaptation to new challenges, and leaving the journal in the best place it has ever been. I have been working with Paul over the past few months to ensure a smooth transition, with the hope of continuing the momentum, growth, and success of our journal.

The list of standard abbreviations for JDS is available at adsa.org/jds-abbreviations-24. Nonstandard abbreviations are available in the Notes.

The milk fatty acid (FA) profile is influenced by complex interactions among animal species, physiology, feeding, and management, resulting in modification to the nutritional and functional properties of milk fat. This study aimed to identify latent biological and nutritional factors influencing milk FA composition in ruminants using multivariate factor analysis (MFA), applied across 4 species: dairy cows (Holstein-Friesian and Brown Swiss), sheep (Sarda and Massese), Mediterranean buffaloes, and goats (Saanen).

Sodium butyrate (SB) is a common feed additive used in calf nutrition to support early growth and gastrointestinal health; however, its long-term programming effects remain poorly characterized. This study examined the dose-dependent effects of preweaning SB supplementation in milk on long-term growth, metabolic profiles, and gastrointestinal microbiota in dairy cattle. Eighty Holstein calves were assigned to one of 4 treatments beginning at 2 to 4 d of age: milk supplemented with 0 (CON), 4.4 (LSB), 8.8 (MSB), or 17.6 (HSB) g/d of SB.

Condensed skim milk was high-pressure jet processed at 100 and 500 MPa and spray dried to produce skim milk powder (SMP). The objective of this study was to improve both the instant and reconstituted physicochemical properties of high-pressure jet spray-dried SMP by introducing an agglomeration step during powder processing. Scanning electron microscopy images verified that effective agglomeration was achieved. Agglomeration with lecithin to SMP processed at 500 MPa improved the wettability time (>60 min to 4 min), sinkability (<1 mg/min.cm2 to 73 mg/min.cm2), and dispersibility (34% to 58%) but not solubility, which remained at 77% (wt/vol).

Genetic evaluations are the cornerstone of modern dairy breeding programs, facilitating selection for traits of economic and environmental importance. The utility of any genetic evaluation is dependent on its ability to predict phenotypic performance in an independent population. This is of particular importance for traits that are not routinely measured on commercial farms, such as feed intake and efficiency. The objective of the present study was to characterize the performance of grazing dairy cows stratified on their EBV for energy intake.

This study evaluated the effects of feeding a probiotic-postbiotic blend on DMI, milk and milk component yields, systemic inflammation, and regional paracellular permeability of the gastrointestinal tract (GIT) before and following an intramammary (IMM) LPS challenge. Lactating Holstein cows (n = 34 at 57 ± 4 DIM) with a SCC <250,000 cells/mL were used, including 14 that were ruminally cannulated. Cows were fed either 28 g/d of a probiotic-postbiotic blend (PB; Dairyman's Edge PRO, Papillon Agricultural Company) or no PB (NP) for 21 d before obtaining 5 d of baseline measurements.

Balancing for limiting AA may prevent reduced production when deficient MP is provided. The objective of the study was to determine the effects of rumen-protected (RP) His supplementation at different levels of MP when Lys and Met supplies meet the requirements. Sixty lactating cows (mean ± SD; parity, 2.47 ± 1.346; DIM, 112 ± 27; milk yield, 50 ± 8 kg/d) were used in a randomized complete block design and allocated to one of the following diets: an MP-adequate diet that meets the MP, Met, Lys, and His requirements (CP, 17.0%) has been reported; an MP-moderately deficient diet (92.6% of the requirement) meeting the requirement of Met and Lys (MDMP; CP, 15.4%); the MDMP diet supplemented with RP-His to meet the His requirement; an MP-deficient diet (86.7% of requirement) meeting the requirement of Met and Lys (DMP; CP, 14.5%); the DMP diet supplemented with RP-His to meet the requirement of His.