A revised version of Figure 5B was sent during author corrections but was not updated in the final article. The correct version of Figure 5 appears below.

Table 3 and Table 4 contained errors in the in situ NDF degradability parameters. The correct parameters appear in boldface below.

The list of standard abbreviations for JDS is available at adsa.org/jds-abbreviations-24. Nonstandard abbreviations are available in the Notes.

The list of standard abbreviations for JDS is available at adsa.org/jds-abbreviations-24. Nonstandard abbreviations are available in the Notes.

Bromoform is a halogenated compound of growing interest due to its potential to reduce methane emissions in dairy cows. This study investigated the partitioning behavior of bromoform during the processing of raw milk that was deliberately dosed with bromoform. Through a pilot-scale study and validated analytical methods, we quantified bromoform concentrations across various product and waste streams and calculated partition coefficients to assess its distribution. Our results showed that bromoform preferentially accumulated in fat-rich matrices, with its concentration closely correlated with the fat content of most product streams.

Previous studies have shown that insufficient phosphorus supply in dairy cows during the transition period can lead to postparturient hemoglobinuria, which is associated with the development of anemia and is presumably a consequence of increased osmotic fragility of erythrocytes. The exact causes of this condition remain unknown. The present study investigated the hypothesis that phosphorus depletion in dairy cows during the transition phase leads to changes in the phospholipid composition of the erythrocyte membrane, which may contribute to increased osmotic fragility.

Supplementing rumen-protected Met (RP-Met) during the transition period has been shown to improve DMI and increase milk production in dairy cows. Omega-3 fatty acids (FA), particularly eicosapentaenoic acid (EPA; C20:5) and docosahexaenoic acid (DHA; C22:6), are known to support immune function in nonruminants, but their effects in dairy cows remain less defined. We aimed to investigate the combined effects of RP-Met and calcium salts (CS) of FA enriched with EPA and DHA on lactation performance in periparturient Holstein cows.

Our objective was to evaluate how feeding colostrum at different volumes at the first feeding affects IgG absorption, gastric emptying, and calf behavior. Female Holstein calves (n = 88) were enrolled in a randomized block design to receive a single colostrum volume corresponding to 6%, 8%, 10%, or 12% of birth body weight (BBW). A subset of animals in 8 blocks (n = 32 calves) also received a gastric emptying marker acetaminophen (Ac) at a dose of 150 mg/kg metabolic body weight (BBW0.75). Colostrum was administered within 2 h of birth via esophageal tube feeder.

Growing evidence demonstrates that the 4-kingdom intestinal microbiota (FKIM) play a critical role in improving the body's immune processes. Three batches of experiments were conducted to study the effects of infant formula–based powder (IFBP) containing milk fat globule membrane (MFGM), human milk oligosaccharides, and probiotics, respectively, on the FKIM of suckling rats, with the IFBP serving as the control. Compared with the control, 3 functional components could increase the body weight and improve the composition of FKIM.

The objective of this study was to evaluate the effects of high-oil oats and cold-pressed rapeseed cake (RSC) as dietary ingredients, along with supplementation of 3-nitrooxypropanol (3-NOP), on apparent total-tract digestibility, milk production, and enteric CH4 emissions in dairy cows fed a grass silage–based diet. Twenty-four lactating Nordic Red cows were grouped into 3 blocks. The experiment was conducted as a cyclic change-over where each treatment had 3 observations per period. The experiment consisted of 4 periods of 28 d each, including 18 d of diet adaptation and 10 d of data and sample collection.

The objective of this study was to investigate Ontario dairy farmers' attitudes and perceptions toward down dairy cows, and the barriers and motivators influencing the use of best management practices. Four focus groups were conducted in southwestern Ontario in 2024, with a total of 21 dairy producers participating. Producers were asked about current practices, thoughts on best management practices, challenges to adopting best practices, and facilities and equipment for down cow care. The focus groups were audio recorded, transcribed, and analyzed using applied thematic analysis.

This study evaluated the effects of propionic acid inclusion (PAI; 0, 0.25, and 0.50% of fresh matter) and ensiling time (ENT; 30, 60, 90, 120, 150, and 180 d) on the chemical composition, fermentation profile, DM losses, and in situ ruminal starch degradability of high-moisture flint corn grain silage (HMC). Rolled corn grain with 45% moisture and 80% vitreousness was ensiled in 10-L mini silos at a packing density of 1,300 kg/m3. The experimental design followed a 3 × 6 factorial arrangement with 3 replicates per treatment, totaling 54 silos.

Heat stress (HS) can affect gut barrier integrity, leading to a leaky gut and elevated enteric bacterial LPS endotoxin levels in the circulation. This LPS can induce systemic inflammation manifesting as the acute-phase response, which includes a febrile response that may compromise heat dissipation from the body during HS. To assess the effects of HS, peripheral blood mononuclear cells (PBMC) were isolated from dairy cows that were previously stress phenotyped based on their 4-h serum cortisol response to Escherichia coli LPS (200 ng/kg administered i.m.).

Heat stress (HS) represents a major threat to the overall welfare and performance of dairy cows; still, genetic evaluations for heat tolerance are not available to dairy producers in the United States. Zoetis has accumulated a large amount of data suitable for genetic and genomic evaluation for heat tolerance. Therefore, the objective of this study was to develop genomic predictions for heat tolerance in US Holstein cattle using milk yield and conception at first service based on producer-recorded data coupled with publicly available weather information.

Listeria monocytogenes is a human pathogen responsible for listeriosis, a sporadic but serious disease that poses significant health risks, particularly to vulnerable populations such as pregnant women, the elderly, and immunocompromised individuals. Mozzarella cheese, a widely consumed fresh dairy product, may represent as a potential vehicle for this pathogen, primarily due to the risk of post-processing contamination. This study presents a systematic review and meta-analysis aimed at estimating the prevalence of L.

Genetic selection to reduce methane production from dairy cows may be an efficient way for reducing the impact of dairy production on climate change. In this study genetic parameters and genomic regions associated with 2 commonly used daily methane features (predicted daily methane emission [PME; g/d]), and log-transformed predicted methane intensity (LMI = log [PME/milk yield (kg)]) were investigated. The PME (g/d) data, predicted using routinely recorded milk mid-infrared spectra, collected between 2007 and 2023 on 285,530 first-parity (1,920,130 test-day records), 224,643 second-parity (1,516,843 test-day records), and 160,226 third-parity (1,072,725 test-day records) Holstein cows distributed in 1,520 herds in the Walloon region of Belgium were used.

Associate milk allowance and starter feed for dairy calves to achieve high performance and adequate nitrogen (N) balance can be challenging. Thus, this study aimed to evaluate the effects of reducing the CP content of the starter feed on the performance, mammary gland development, and N balance of dairy calves in the preweaning, weaning, and postweaning phases. The study was conducted according to a completely randomized design in a 3 × 2 factorial scheme, using 3 milk volumes (4, 6, or 8 L/d) and 2 strategies for starter feed supply (fixed or decreasing CP content).

The objective of this study was to estimate the costs associated with raising dairy calves and heifers in Brazil during 2021. Additionally, it sought to determine the average number of lactations that heifers should remain in the herd to cover their production costs, analyzing data from 311 dairy farms in Minas Gerais. These farms were categorized into 3 milk productive tiers based on daily milk production per cow: lower production tier (LOW) 12.0 L/cow per day (7.4–14.5 L/cow per day); intermediate production tier (INT) 18.0 L/cow per day (14.6–22.5 L/cow per day), and upper production tier (UPP) 26.7 L/cow per day (22.6–32.0 L/cow per day).

Individual housing of dairy calves has received criticism due to animal welfare concerns. Therefore, it is important to provide researchers and practitioners with accurate and reliable scientific knowledge on alternative housing systems for dairy calves. Our objective was to critically evaluate the scientific knowledge on pair-housed dairy calves. The outcomes investigated were the performance, behavior, reactivity to stress, health, learning abilities, and emotional states of pair-housed calves.

Metritis and mastitis are common early-lactation diseases of dairy cows that reduce milk production. Early prediction enables timely intervention and management, yet no studies have investigated the ability of milk Fourier-transform infrared (FTIR) spectroscopy for predicting the onset and development of metritis or mastitis within the first 2 wk postpartum. Our study aimed to assess the potential of milk FTIR spectra for early detection of postpartum metritis and clinical mastitis and to describe their spectral variations as lactation advances and diseases progress.