Whey protein is widely recognized as a high-quality protein source, primarily derived from dairy products. This type of protein is valued not only for its remarkable nutritional benefits but also for its substantial antioxidant properties and its capacity to reduce inflammation. Previous studies have demonstrated that whey protein hydrolysate (WPH) is effective in both the prevention and treatment of liver injury caused by acetaminophen (APAP) overdose. Pyroptosis is a form of programmed cell death that significantly contributes to liver damage induced by APAP, and it is associated with increased levels of oxidative stress and inflammation.

The aim of this work was to determine how the addition of hydrolyzed walnut oil, types of lipases (from Candida rugosa and porcine pancreas) and storage time affect the composition, acidity, microbiota, free fatty acids and fatty acids content of sour milk and yogurt plus the monocultures of Lactobacillus acidophilus LA-5 and Bifidobacterium animalis ssp. lactis BB-12. Lipases were used to release free form of linoleic acid (LA) from walnut oil to create conditions favorable for conjugated linoleic acid (CLA) synthesis by the starter culture.

Whether lactose or fat is a better fuel for protein synthesis and deposition in young calves has remained controversial. Also, it remains uncertain whether bioactive factors in whole milk might influence growth compared with a milk replacer of similar macronutrient composition. We used Holstein calves (3 wk old, 46.0 kg BW) to determine effects of energy source on growth and body composition over a 35-d experiment. Calves (n = 29) were assigned either to an initial body composition group (n = 11) or to 1 of 3 treatment groups (n = 6 per group).

The effect on pregnancy per artificial insemination (P/AI) of interval from induction of ovulation to timed artificial insemination (TAI) for cows submitted to a fertility program and the interval from the onset of estrous alert to artificial insemination (AI) for cows inseminated to estrous alert was analyzed in 3 experiments. In experiment 1, multiparous lactating Holstein cows (n = 1,924) from 6 herds were submitted to a Double-Ovsynch protocol to receive their first TAI using conventional semen.

Penetrating captive bolt (PCB) is a method of euthanasia and pre-slaughter stunning for cattle. Industry guidelines include multiple PCB locations with minimal scientific justifications. Objectives were to determine tissue depths, PCB position relative to anatomic features, and brain contact associated with 5 frontal placements and a poll placement for mature dairy cows. Cadaver heads were obtained from 22 Holstein cows >30 mos of age (HCW, mean ± SD: 278.83 ± 46.61 kg); all heads were stunned at a frontal location with a PCB at the collaborating slaughter establishment.

Obesity driven by high-fat diets is associated with metabolic dysregulation and gut microbiota disruption. Traditional fermented dairy products, such as Qula from Qinghai Province, China, are valuable sources for isolating novel probiotics with potential therapeutic applications against obesity. This study explored the appetite-regulating mechanisms and gut microbiota modulation by Lacticaseibacillus rhamnosus HF01, a strain isolated from traditional Qula, a type of dairy product in Qinghai Province, China, in mice with high-fat diet–induced obesity.

The Lacticaseibacillus paracasei ProSci-92 (ProSci-92) strain was isolated from naturally fermented sour camel milk in the Alxa League of Inner Mongolia Autonomous Region, China. Initial screening revealed its promising potential for lactose and protein hydrolysis. This study monitored the growth and metabolic dynamics of ProSci-92 and during a 60-h milk fermentation process using non-targeted metabolomics. Fermented milk samples were collected every 12 h, enabling comparative metabolomic profiling at adjacent time points.

Narrow-leaved plantain (Plantago lanceolata L.) has been proposed as a potential solution to sub-surface drainage (i.e., leaching) losses of soluble nitrogen (N) species (primarily nitrate; NO3-), a critical environmental challenge in grazing systems. It has been hypothesized that plantain has properties that can lower NO3- leaching through animal, plant, and soil-related processes. We summarize available evidence, explore mechanistic bases, and investigate the integration of plantain into mixed forage pastures.

Several reproductive disorders can occur in dairy cows from peri-partum until the start of pregnancy. Their occurrence can be interrelated, which complicates the estimation of subsequent economic impact. Estimation of the economic impact of reproductive disorders is essential for dairy farmers to make informed decisions. It enables them to prioritize the prevention of disorders with the highest economic impact. The aim of this study was to estimate the costs of dairy cow reproductive disorders, including dystocia, retained placenta, acute metritis, clinical endometritis, anovulation, cystic ovarian disease and sub-estrus, taking their complex interrelations into account.

The objective of the study was to quantify how non-genetic factors associated with gestation length; also of interest was the associations between gestation length and calving performance. The study comprised 9,750 gestation length records from 4,256 multiparous dairy cows as few breeding dates were available for heifers. A linear mixed model was used to investigate factors associated with gestation length, while logistic regression was used for the analysis of perinatal mortality (including still births), calving assistance and dystocia; calf birth weight and gestation length estimated breeding values (EBV) were included as covariates in some models.

Mid-infrared spectroscopy (MIRS) is increasingly used as a rapid and effective analytical method for the quantitative prediction of detailed milk composition, such as minerals, fatty acids, and amino acids (AA). These analyses require the transportation of samples to a certified laboratory. In this case, storage time may affect MIRS and its prediction results. This study aimed to determine the effect of milk storage time on MIRS and its predictions of AA content. 373 individual milk samples for the development of AA content prediction equations were collected from 7 commercial dairy farms (data set 1).

Ready-to-drink protein beverages are a growing product category. The objective of this study was to determine the effects of 3 common thermal processes: UHT pasteurization with direct steam injection (UHT-DSI; 142°C for 3 s), retort (123°C, F0 = 5), or autoclave (112°C for 6 min) on the chemical, physical, and sensory properties of reconstituted vanilla-flavored milk protein beverages containing approximately 6.5% protein. Unheated and heated beverages were subjected to composition, minerals, pH, color, apparent viscosity, particle size, trained panel profiling, volatile compound analysis, and furosine analyses.

Specialty milks, known for their distinct nutritional profiles and expensive pricing, have received increased attention as a result of worries over their adulteration with cow milk. This study used capillary electrophoresis to detect cow milk adulteration (2%, 5%, 10%, 30%, and 50%) in raw, pasteurized, freeze-dried, and spray-dried forms of 7 specialty milks (buffalo, yak, goat, sheep, camel, horse, and donkey) and to identify particular markers derived from cow components. Cow αS1-casein, β-casein A1, β-lactoglobulin, and α-lactalbumin were identified as potential markers for detecting cow milk adulteration in specific milk types, based on their retention times.

Kefir is an increasingly popular dairy- or sugar-based fermented food product. The aim of our study was to investigate the bacterial and fungal communities in 28 retail kefirs including 21 milk kefirs, including 3 thick kefir yogurt and 4 water kefir products. Full-length amplicon nanopore sequencing of both 16S rRNA (for bacteria) and intergenic spacer (for fungi) was undertaken. The diversity within and between groups was analyzed (α and β diversity) and linear discriminant analysis effect size analysis was undertaken to identify biomarkers that differentially characterize the microbial communities associated with different kefir types.

Small RNAs (sRNAs) play crucial roles in response to environmental stress and contribute to bacterial survival. However, the understanding of sRNA response and regulatory mechanisms in Lactococcus lactis remains limited. In this study, a novel acid-activated sRNA, C263, was identified, which enhances cell wall peptidoglycan synthesis by base pairing with the mRNA 5′ untranslated region (5′UTR) of murA, murC, and mraY. Additionally, it was observed that transcriptional regulator RmaE can activated sRNA C263 through binding to its promoter region at 5′-AAAATTTT-3′.

This study aimed to describe the effect of a potassium sorbate preservative on bacterial proliferation in bovine colostrum and milk, and on colostrum pH and IgG concentrations, when stored at refrigeration (RF) or room temperature (RT) for 96 h. Fifteen batches of previously frozen raw colostrum and bulk tank milk (BTM) were inoculated with Escherichia coli, Salmonella enteritidis, and Staphylococcus aureus. Each batch was divided into 2 aliquots, with one kept as an untreated control (CON) and the other treated with a liquid potassium sorbate preservative (PSP) to achieve a 0.3% and 0.15% solution for colostrum and milk, respectively.

Understanding the biology underlying variation in feed efficiency is crucial for improving nutrient use efficiency at the tissue and molecular level in dairy cows. This study aimed to identify key differences in hepatic nutrient metabolism by analyzing protein-level alterations through proteomics and quantifying associated changes in circulating AA levels between cows with high- or low-residual feed intake (RFI). Ninety-two mid-lactation (119 ± 33 d in milk) primi- (n = 34) and multi-parous (n = 58) Holstein cows were enrolled in 42-d studies in 2 cohort groups.

During the production of dairy-based drinks or their early storage months, there are no established chemical or physical indicators to reliably predict whether a specific beverage will remain stable throughout its intended shelf life or undergo premature destabilization. Consequently, the industry relies on observing physical changes, such as increased viscosity or particle size, to assess the shelf stability of milk protein concentrate–based low-acid beverages. This study aims to evaluate the effectiveness of the electrical resistance tomography in liner configuration in detecting destabilization in high-protein beverages (HPB).

The objective of this study was to determine if a commercial feed additive blend composed of quebracho, chestnut tannins, and saponins (TAN; Silvafeed BX) could reduce enteric GHG and ammonia (NH3) emissions without negatively effecting the productive performance of dairy cows. Twenty mid lactation Holstein dairy cows were blocked by DIM and parity in a randomized complete block design, and were assigned to 1 of 2 treatments: TAN (n = 10) or control with no TAN (CON; n = 10). The treatments were administered as a top dress at each of 2 daily feedings with TAN supplemented at a rate of 0.07% of dietary DM.

Dominant species common to the kefir grains from 7 different origins were analyzed using culture-dependent methods using agar media that were able to grow and discriminate Lactobacillus kefiranofaciens ssp. kefiranofaciens. Identification of all isolates was performed on the basis of MALDI-TOF MS analysis and colony morphology. Lactic acid bacteria were present at 9 log cfu g−1 in all kefir grains. Lactobacillus kefiranofaciens was the predominant species (ca. 87%), followed by Lentilactobacillus kefiri (ca.